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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
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Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal fibroblasts (HDF) after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).

Journal: Polymers

Article Title: Amylopectin- g -Poly(Acrylic Acid): Synthesis and Application as Reduction Agent for In Situ Formation of Gold Nanoparticles

doi: 10.3390/polym18131636

Figure Lengend Snippet: Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal fibroblasts (HDF) after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).

Article Snippet: The cytotoxicity of both the AMP- g -PAA copolymer and the AMP- g -PAA/AuNPs composites was evaluated using a Human dermal fibroblasts (HDF) cell line from Innoprot (Derio, Spain).

Techniques: Cell Viability Assay, Negative Control, Standard Deviation, Control